The presence of the drug resistance plasmid pKM101 restored the ability of Escherichia coli umuC mutant strains to be mutated by methyl methanesulfonate. Inducible (Weigle) reactivation of ultraviolet-irradiated bacteriophage lambda was not observed in uvrA6 umuC mutant strains lacking pKM101 but wa
Expression of the plasmid pKM101-determined DNA repair system in recA − and lex − strains of Escherichia coli
✍ Scribed by Monti-Bragadin, C. ;Babudri, N. ;Samer, L.
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 292 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
pKM101, a plasmid R factor of the N compatibility group increases methylmethane sulfonate mutagenesis and diminishes UV-killing in recA+ LEX+ and recA+ lex- strains, , but not in recA-lex+ strains. The induction of a "reclex" dependent colicin is not present in lex- strains carrying the pKM101 factor. These facts indicate that pKM101 acts through an error-prone DNA repair system which is recA+ dependent, but not lex+ dependent.
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