Expression of the murine interleukin 6 receptor in hepatoma cells: the intracytoplasmic domain is not required for interleukin 6 signal transduction

✍ Scribed by Maria Teresa Fiorillo; Carlo Toniatti; Jacques Van Snick; Gennaro Ciliberto

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 604 KB
• Volume: 22
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830220325

✦ Synopsis

This paper reports on cDNA coding for the SO-kDa murine IL6 receptor (mlL6R) that was cloned from a mouse liver cDNA library. Human hepatoma Hep3B cells transfected transiently or stably with an expression vector carrying the entire coding region for mIL6R become responsive to mouse IL6 (mIL 6).We monitored response to the cytokine through the transcriptional activation of a co-transfected IL 6-inducible human C-reactive protein (CRP) promoter; response to mIL 6 is lost upon treatment of the cells with increasing amounts of a monoclonal antibody to mIL6R. mIL6R mutants have been generated in the carboxy-terminal portion of the molecule. Their functional analysis in hepatoma cells shows that the intracytoplasmic domain of the receptor is not absolutely essential to IL6 signal transduction (ie. CKP promoter activation), but that the last 40 amino acids contribute to maximal IL6 response in these cells.