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Expression of the murine complement regulatory protein Crry by glial cells and neurons

✍ Scribed by Nathalie Davoust; Serge Nataf; V. Michael Holers; Scott R. Barnum

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 371 KB
Volume: 27
Category: Article
ISSN: 0894-1491
DOI: 10.1002/(sici)1098-1136(199908)27:2<162::aid-glia6>3.0.co;2-k

No coin nor oath required. For personal study only.

✦ Synopsis

The expression of the murine complement regulatory protein, Crry, in the CNS remains largely unexplored. In this study, we examined murine astrocytes and microglia purified from neonatal brain and sections of adult murine brain for the expression of Crry. Using RT-PCR, immunohistochemistry, in situ hybridization, flow cytometry, and Western blot analysis, we demonstrated that astrocytes and microglia express Crry protein and RNA. Crry expression is greater on microglia than astrocytes and, as determined by Western blot analysis, each cell type expresses a Crry protein of different molecular weight. Interestingly, neuronal expression of Crry was seen only at the RNA level. These data demonstrate Crry expression by astrocytes, microglia, and neurons in the murine CNS and suggest that Crry may play an important role in protecting the CNS against complement-mediated damage.

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