EXPRESSION OF THE LAMININ γ2 CHAIN IN PANCREATIC ADENOCARCINOMA

✍ Scribed by SOINI, YLERMI; MÄÄTTÄ, MARKO; SALO, SIRPA; TRYGGVASON, KARL; AUTIO-HARMAINEN, HELENA

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 600 KB
• Volume: 180
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199611)180:3<290::aid-path661>3.0.co;2-6

✦ Synopsis

Forty-two pancreatic adenocarcinomas were investigated immunohistochemically and by in situ hybridization for the expression of the laminin 72 chain. In 41 cases, intracytoplasmic immunoreactivity for the y 2 chain was seen. Positive tumour cells were located especially at the epithelial-stromal interface of the tumour cell islands. In 22 cases, diffuse laminin y 2 chain immunoreactivity could also be seen in stroma and in seven cases, occasional positivity was detected in the neoplastic basement membranes. Signals for laminin y 2 chain mRNA in tumour cells displayed a distribution similar to that observed on immunohistochemistry. There were significantly more cases with less than 20 per cent of laminin y 2 chain-positive tumour cells in tumours extending to peripancreatic tissues andlor tumours with regional or distant metastases (P=0*029). A corresponding statistical significance could also be noted in the mRNA level (P=0*025). The results show that pancreatic adenocarcinomas display a high activity of laminin y 2 chain synthesis. Tumours with a strong laminin y 2 chain synthesis show a lower invasive and metastatic potential than tumours with a weak or moderate laminin 72 chain expression.