Expression of the laminin ?2 chain in different histological types of lung carcinoma. A study by immunohistochemistry andin situ hybridization
✍ Scribed by M��tt�, Marko; Soini, Ylermi; P��kk�, Paavo; Salo, Sirpa; Tryggvason, Karl; Autio-Harmainen, Helena
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 545 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
Sixty-four malignant lung tumours and 12 of their regional lymph node metastases were analysed for expression of the laminin 2 chain by immunohistochemistry and in situ hybridization. Expression of the laminin 2 chain was strongest in squamous cell carcinomas, followed by adenocarcinomas and large cell carcinomas. Positive cells, except for large cell carcinomas, were located at the epithelial-stromal interface of tumour clusters. An important exception was small cell lung carcinoma, with only a low level of laminin 2 chain expression. Apart from tumour type, this may reflect the relatively scanty fibrous stroma in these tumours and supports previous observations that small cell lung carcinoma cells, contrary to other types, lack surface expression of 6 4 integrin, the specific laminin-5 binding receptor. In frozen sections, immunohistochemistry showed linear basement membranes around tumour clusters in squamous cell carcinomas and adenocarcinomas. This shows that carcinoma cells are capable of heavy deposition of the laminin 2 chain around tumour clusters and suggests that a laminin 2 chain-containing substrate may be of significance for the spread and growth of malignant tumours.