Expression of the DX5 antigen on CD8+ T cells is associated with activation and subsequent cell death or memory during influenza virus infection
✍ Scribed by Taku Kambayashi; Erika Assarsson; Benedict J. Chambers; Hans-Gustaf Ljunggren
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 181 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The antigen recognized by the DX5 antibody (DX5 antigen) is expressed on all murine NK cells. In the present study we found that a proportion of CD8 + T cells ( ˚5%) also express the DX5 antigen in uninfected mice, and that numbers of CD8 + T cells expressing DX5 are significantly higher in the lungs of influenza virus-infected mice representing up to 50% of all CD8 + T cells on day 10 post infection. The expression of the DX5 antigen on CD8 + T cells was associated with a memory phenotype in uninfected C57BL/6 mice and with an activation phenotype during influenza virus infection. Interestingly, when lymphocytes were isolated from lungs of influenza virus-infected mice on day 10 post infection and adoptively transferred into recombination activating gene-1 (RAG1)-deficient mice, CD8 + DX5 + cells could not be recovered from the recipient mice 2 days later. Moreover, CD8 + DX5 + cells were not detected when lung cells were removed from day 10 influenza virus-infected mice and cultured in vitro for 2 days. However, CD8 + DX5 + cells could be detected when apoptosis inhibitors were added to these cultures, suggesting that the CD8 + DX5 + cells underwent apoptosis during cell culture. Furthermore, almost all DX5 expressing CD8 + cells from lungs of mice on day 10 post influenza virus infection stained positively with Annexin-V. Taken together, the data suggest that CD8 + T cells expressing DX5 are associated with an activation/memory phenotype and are biased towards apoptosis.