Expression of TGF-β and procollagen type I and type III in human gastric carcinomas

✍ Scribed by Kazuhiro Yoshida; Hiroshi Yokozaki; Minoru Niimoto; Hisao Ito; Masanori Ito; Eiichi Tahara

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 940 KB
• Volume: 44
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910440303

✦ Synopsis

To elucidate the difference between xirrhous and nonxirrhous gastric Carcinomas, we examined the expressions of TGF-P, procollagen type I and type 111 in 7 gastric carcinoma cell lines and 37 gastric carcinoma tissues, and also examined the effect of TGF-P on the expression of procollagen mRNA by TMK-I cells. TGF-P mRNA was detected in all the tumors examined in vivo and in vitro. Interestingly, 9 (90%) of 10 scirrhous gastric carcinomas revealed higher levels of TGF-P mRNA than normal tissues, while 8 (38%) of 21 welldifferentiated adenocarcinomas had higher TGF-P mRNA levels than normal tissues. As for procollagen mRNA, most of the human gastric carcinoma cell lines expressed type-I procollagen mRNA and MKN-I expressed type-Ill procollagen mRNA. Furthermore, procollagen type-I mRNA accumulation in TMK-I cells was increased by exogenous TGF-P. Most of the tumor tissues from surgical specimens expressed higher procollagen mRNA than normal tissues. These results indicate that TGF-P produced by carcinoma cells might stimulate collagen synthesis not only by fibroblasts but also by carcinoma cells themselves, leading to diffuse fibrosis of scirrhous gastric carcinomas.