Expression of Spicule Matrix Proteins in the Sea Urchin Embryo during Normal and Experimentally Altered Spiculogenesis
β Scribed by Lisa A. Urry; Patricia C. Hamilton; Christopher E. Killian; Fred H. Wilt
- Book ID
- 115598529
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 421 KB
- Volume
- 225
- Category
- Article
- ISSN
- 0012-1606
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Studies of the sea urchin larval skeleton have contributed greatly to our understanding of the process of biomineralization. In this study we have undertaken an investigation of the morphology of skeleton formation and the localization of proteins involved in the process of spicule form
We have purified and characterized a collagenase/gelatinase activity expressed during sea urchin embryonic development. The native molecular mass was determined to be 160 kDa, while gelatin substrate gel zymography revealed an active species of 41 kDa, suggesting that the native enzyme is a tetramer