Expression of sodium-dependent phosphate (NadPi) transport inXenopus laevisoocytes induced by mRNA from 1α,25-dihydroxyvitamin D3-treated rat osteoblast-like cells
✍ Scribed by Christian M. Veldman; Daniel Markovich; Christoph Schmid; Heini Murer
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 435 KB
- Volume
- 430
- Category
- Article
- ISSN
- 0031-6768
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✦ Synopsis
Injection of messenger ribonucleic acid (mRNA) isolated from l~,25-dihydroxyvitamin D3treated osteoblast-like (PyMS) cells leads to an enhanced sodium-dependent phosphate (NadPi) transport in Xenopus laevis oocytes, when compared to untreated cells. After mRNA size fractionation, mRNA with an average size of 2.2-3.8 kilobases showed up to a 1.8-fold stimulation of NadPi transport encoding either directly a NaaPi transporter(s) or proteins controlling their activity. No hybridization was observed in Northern blots with RNA from rat bone or PyMS cells with the recently cloned rat renal brush border NaaPi transporter NaPi-2; hybrid depletion with a NaPi-2 antisense oligonucleotide did not abolish the PyMS mRNA-induced NadPi transpor t in oocytes. We present the first evidence for functional expression in Xenopus laevis oocytes of a new type of NadPi transport system in bone cells, which is different from the renal type.