Expression of scfv antibodies in xenopus embryos to disrupt protein function: Implications for large-scale evaluation of the embryonic proteome
✍ Scribed by Lisa L. Abler; Michael D. Sheets
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 269 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1526-954X
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✦ Synopsis
Abstract
Summary: We evaluated the use of single‐chain antibody (scFv) expression as a tool to disrupt the function of specific proteins in embryos of the frog, Xenopus laevis. The expression of scFvs that recognize the bone morphogenetic protein receptor (ALK3) or the fibroblast growth factor receptor1 (FGFR1) as endoplasmic reticulum‐anchored proteins caused distinct developmental defects that were virtually indistinguishable from the defects caused by expression of the dominant negative forms of each receptor. These results demonstrate that scFvs from phage‐display libraries can be readily fashioned into effective and specific inhibitors of signaling pathways in developing embryos. In addition, as several effective scFvs against a specific target can be isolated rapidly, this approach represents a valuable new tool for large‐scale functional analysis of the embryonic proteome. genesis 35:107–113, 2003. © 2003 Wiley‐Liss, Inc.