Expression of recombinant DNA functional products in Escherichia coli anucleate minicells
โ Scribed by Khachatourians, George G.; Berezowsky, Craig M.S.
- Book ID
- 122428128
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 593 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0734-9750
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The properties of minicell producing mutants of Escherichia coli deficient in gentic recombination were examined. Experiments were designed to test recombinant formation in conjugal crosses, survival following UV-irradiation in cells, and the state of DNA metabolism in minicells. The REC- phenotypes
A genetic strategy to enhance recombinant protein production is discussed. A small DNA bending protein, Fis, which has been shown to activate rRNA synthesis upon a nutrient upshift, was overexpressed in E. coli strain W3110 carrying vector pUCR1. Overexpres- sion of Fis during exponential growth was