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Expression of rat diamine oxidase in Escherichia coli

✍ Scribed by Elena Rosini; Serena Nossa; Mattia Valentino; Paola D’Arrigo; Stéphane Marinesco; Loredano Pollegioni

Book ID
116753491
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
555 KB
Volume
82
Category
Article
ISSN
1381-1177

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Identification of Topaquinone, As Illust
Identification of Topaquinone, As Illustrated for Pig Kidney Diamine Oxidase and Escherichia coli Amine Oxidase
✍ V. Steinebach; B.W. Groen; S.S. Wijmenga; W.M.A. Niessen; J.A. Jongejan; J.A. Du 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 592 KB

Pig kidney diamine oxidase was purified to homogeneity. The reaction product of the cofactor with p-nitrophenylhydrazine (pNPH) was liberated with pronase treatment and purified. 1H NMR, uv/vis, and electrospray tandem mass spectroscopy revealed it to be a dipeptide with the sequence topaquinone-pNP