Expression of post-transcriptional regulatory gene of HTLV-I,rex, inEscherichia coli

✍ Scribed by Zhi-Ai Zhou; Yoshifumi Adachi; Masatoshi Maki; Masakazu Hatanaka

• Book ID: 104631601
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 603 KB
• Volume: 3
• Category: Article
• ISSN: 0920-8569
• DOI: 10.1007/bf00125127

✦ Synopsis

Human T-cell leukemia virus type-1 (HTLV-I) has a post-transcriptional regulatory gene termed rex. We have designed the rex gene to express in E. coli. Synthesis of rex protein, p27reX, was examined by immunoblot analysis using anti-~27~~~ antibody. No difference in electrophoretic mobility in NaDadSOa-PAGE was observed between ~27~~ expressed in E. co/i and in an HTLV-l-infected cell line, MT-2. Slower migration of ~27~~', corresponding to a 27-kD protein, in NaDodSOd-PAGE when compared with the calculated molecular weight from the amino acid sequence (Mr= 20,367) is suggested to be caused not by posttranslational modification, but by the intrinsic nature of the protein, which is rich in prohne and arginine.