Expression of p34cdc2 and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: A multivariate cluster analysis

✍ Scribed by Lorenzo Leoncini; Antonio Cossu; Tiziana Megha; Cristiana Bellan; Stefano Lazzi; Pietro Luzi; Piero Tosi; Paolo Barbini; Gabriele Cevenini; Stefano Pileri; Antonio Giordano; Rainer Kraft; Jean A. Laissue; Hans Cottier

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: French
• Weight: 172 KB
• Volume: 83
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19991008)83:2<203::aid-ijc10>3.0.co;2-0

✦ Synopsis

In view of recent knowledge on proteins regulating the cell cycle, we re-evaluated proliferative features of 98 diffusely growing non-Hodgkin's lymphomas. The combined use of 5 proliferation-associated variables (mitotic indices and percentages of Ki-67 ؉ , p34 cdc2؉ , cyclin A ؉ and cyclin B ؉ cells) and their entry into a multivariate cluster analysis separated, without overlaps, the entire cohort into 3 groups (clusters) with (1) low, (2) intermediate and (3) high proliferative activity. Conversely, bivariate plots exposed considerable cluster overlaps. Multivariate stepwise discriminant analysis of all cases revealed a decreasing order of discriminant power for % Ki-67 ؉ cells G % p34 cdc2؉ cells G mitotic index G % cyclin A ؉ cells G % cyclin B ؉ cells. The combined use of 2 variables only, mitotic index and % p34 cdc2؉ cells, allowed a clear-cut separation of clusters 2 and 3. In bivariate plots, correlations were best between % Ki-67 ؉ cells and % cyclin A ؉ cells and between mitotic indices and % cyclin B ؉ cells. Except for chronic lymphocytic leukemias, immunocytomas and marginal zone lymphomas (all in cluster 1), individual lymphoma entities were distributed among at least 2 clusters. There was, however, a marked preponderance of mantle cell lymphomas and diffuse follicular center lymphomas in cluster 1 and of diffuse large B-cell lymphomas and peripheral T-cell lymphomas in cluster 2. Anaplastic large-cell lymphomas predominated in cluster 3 and responded best to therapy. Int.