Expression of Nox4 in osteoclasts

✍ Scribed by Su Yang; Yizhong Zhang; William Ries; Lyndon Key

• Book ID: 102304815
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 245 KB
• Volume: 92
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.20048

✦ Synopsis

Abstract

A new superoxide‐generating enzyme, NADPH oxidase 4 (Nox4), contributes to osteoclastic superoxide production. In this study, we demonstrated that Nox4 is expressed at a higher level in osteoclasts than that in precursor cells. This result suggested that Nox4 is upregulated during the differentiation and development of osteoclasts. Cotransfection of Nox4/P22 DNA resulted in enhanced superoxide production in osteoclasts, indicating that P22 may be a necessary factor for the Nox4 activity. In addition, expression of both cathepsin K and TRAP is increased significantly in osteoclasts cotransfected with Nox4/P22. Further study revealed that JNK was activated and that NF‐κB was inhibited in Nox4/P22 cotransfected osteoclasts. These findings suggest that superoxide and/or superoxide derived molecules may modulate the signal transduction pathways necessary for osteoclasts to function. © 2004 Wiley‐Liss, Inc.