## Abstract A clone of a fast isoform of myosin heavy chain (HC) gene was isolated from a cDNAThe sequence data for this cDNA has been submitted to GenBank; the accession number is U03091. expression library made from mRNA purified from the deep abdominal flexor muscle of the lobster, __Homarus am
Expression of myosin heavy chain isoforms in myogenic clones obtained from developing quail breast muscle
β Scribed by Latham, Keith E. ;Konigsberg, Irwin R.
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 692 KB
- Volume
- 197
- Category
- Article
- ISSN
- 1432-041X
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β¦ Synopsis
Monoclonal antibodies specifically recognizing cardiac and extraocular muscle myosin heavy chains of the quail (Coturnix coturnix japonica) were used to determine the patterns of expression of these isoforms in clonal cultures of embryonic quail myoblasts. Myoblasts prepared from 9 day embryonic pectoralis are virtually homogeneous in their ability to form clones expressing both cardiac and extraocular isoforms. The majority of myoblasts obtained from day 5 embryos also formed clones which co-express the cardiac and extraocular isoforms, but a small percentage of the clones expressed only cardiac isoforms.
π SIMILAR VOLUMES
Myosin heavy chains (hcs) are the major determinant in the speed of contraction of skeletal muscle, and various isoforms are differentially expressed depending on the functional activity of the muscle. Using the rapid amplification of cDNA ends (3Π RACE) method, we have characterised the 3Π end of t