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Expression of MRP and mdr1 in human gastrointestinal cancer cell lines: A correlation with resistance against doxorubicin

✍ Scribed by Murase, Masaharu; Kodera, Yasuhiro; Kondo, Ken; Sekiguchi, Hiroyuki; Fujiwara, Michitaka; Kasai, Yasushi; Akiyama, Seiji; Ito, Katsuki; Takagi, Hiroshi

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
596 KB
Volume
61
Category
Article
ISSN
0022-4790

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✦ Synopsis

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The mRNA expression of mdrl and MRP, each of which codes for a transport protein belonging to ATP-binding cassette superfamily and are reported to be responsible for multidrug resistance phenotype, were semiquantified by RT-PCR in a panel of gastrointestinal cancer cell lines.

Although the expression of MRP was predominant in esophageal cancer cell lines, expression of either or both of the genes was detected in all the cell lines tested. Expression of these two genes added together correlated significantly with chemosensitivity against doxorubicin, implicating that expression of both genes should be evaluated in the future analysis of multidrug resistance phenotype. The IDSo values for pirarubicin, although generally lower than the values for doxorubicin, correlated well with the latter, suggesting that the similar phenotype as that for doxorubicin might be responsible for drug resistance against this semisynthetic anthracycline glycoside.

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