Expression of Micro-RNA-145 is regulated by a highly conserved genomic sequence 3′ to the pre-miR
✍ Scribed by Gaspare La Rocca; Bin Shi; Laura Sepp-Lorenzino; Renato Baserga
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 366 KB
- Volume
- 226
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Micro‐RNA‐145 (miR145), a tumor suppressor miR, dramatically inhibits growth of cancer cells in culture and plays a significant role in human stem cells differentiation. We have isolated a human genomic sequence of 864 bp comprising the pre‐miR and its flanking sequences. The cloned miR145 genomic sequence expresses a mature miR145 in transfected cells. We show here that flanking sequences on either side of the pre‐miR sequence can modulate its expression levels. Surprisingly, a highly conserved sequence 3′ to the pre‐miR plays a crucial role in miR145 expression. J. Cell. Physiol. 226: 602–607, 2011. © 2010 Wiley‐Liss, Inc.