Expression of matrix metalloproteinase-1, -7, -9, -13, Ki-67, and HER-2 in epithelial-myoepithelial salivary gland cancer

✍ Scribed by Heikki Luukkaa; Pekka Klemi; Ilmo Leivo; Antti A. Mäkitie; Jonathan Irish; Ralph Gilbert; Bayardo Perez-Ordonez; Pirkko Hirsimäki; Tero Vahlberg; Atte Kivisaari; Veli-Matti Kähäri; Reidar Grénman

• Book ID: 102847595
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 531 KB
• Volume: 32
• Category: Article
• ISSN: 1043-3074
• DOI: 10.1002/hed.21277

✦ Synopsis

Abstract

Background

The expression of matrix metalloproteinases (MMPs) in epithelial‐myoepithelial salivary gland carcinoma has not been studied previously.

Methods

Immunohistochemistry for MMP‐1, ‐7, ‐9, ‐13, Ki‐67, and HER‐2, as well as HER‐2 gene amplification by silver enhanced in situ hybridization was performed in a series of 12 paraffin‐embedded histopathologic samples of patients from Canada and Finland.

Results

A positive MMP‐13 (p = .0022), higher MMP‐13 (p = .0274), and higher MMP‐9 (p = .0274) index (multiplication of staining intensity by percentage of the positive cells) predicted better overall survival. In disease‐specific analysis, higher MMP‐9 index (p = .0327) predicted better survival. A higher volume corrected index (VCI) of Ki‐67 (p = .0339) predicted worse disease‐specific survival. In 1 patient, HER‐2 oncogene amplification was observed.

Conclusion

MMPs and Ki‐67 may have prognostic impact in epithelial‐myoepithelial carcinoma. © 2009 Wiley Periodicals, Inc. Head Neck, 2010