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Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells

✍ Scribed by Victoria Sarafian; Michel Jadot; Jean-Michel Foidart; Jean-Jacques Letesson; Frédéric Van den Brûle; Vincent Castronovo; Robert Wattiaux; Simone Wattiaux-De Coninck

Publisher
John Wiley and Sons
Year
1998
Tongue
French
Weight
305 KB
Volume
75
Category
Article
ISSN
0020-7136

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✦ Synopsis

Lysosomal-membrane-associated glycoproteins (Lamps) 1 and 2 are rarely found on the plasma membranes of normal cells. There is evidence suggesting an increase in their cellsurface expression in tumor cells, with some data indicating that the adhesion of some cancer cells to the extracellular matrix is partly mediated by interactions between Lamps and E-selectin and between Lamps and galectins (endogenousgalactoside-binding lectins). The present study examined the expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in different human tumor cell lines. Indirect immunofluorescence staining revealed accumulation of Lamp molecules at the edges of A2058 human metastasizing melanoma cells suggesting that these glycoproteins could participate in cell adhesion. Flow cytometry showed the presence of cellsurface Lamps in A2058, HT1080 (human fibrosarcoma) and CaCo-2 (human colon-adenocarcinoma) cells. Treatment with 2 mM sodium butyrate for 24 and 48 hr resulted in a significant increase in Lamps surface expression. A strong binding of A2058 to recombinant galectin-3 was detected by FACS. The application of 2 and 5 mM butyrate for the same incubation period enhanced galectin-3 binding to Lampsexpressing cells. Our results support the idea that Lamps may be considered a new family of adhesive glycoproteins participating in the complex process of tumor invasion and metastasis.

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