Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter
β Scribed by CANNON, F. C.; POSTGATE, J. R.
- Book ID
- 109698632
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 219 KB
- Volume
- 260
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/260271a0
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The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of h
Some restriction endonuclease fragments of nif DNA, when carried on small multicopy plasmids, inhibited nif expression in Klebsiella pneumoniae. A study of this inhibitory effect revealed, (1) that overproduction of the nifL gene product inhibited transcription of two nif operons examined, nifJ and