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Expression of insulin-like growth factor binding proteins in human breast cancer correlates with estrogen receptor status

โœ Scribed by Jose A. Figueroa; James G. Jackson; William L. McGuire; Robert F. Krywicki; Douglas Yee

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
875 KB
Volume
52
Category
Article
ISSN
0730-2312

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โœฆ Synopsis

The insulin-like growth factors (IGFs) have been implicated in the growth regulation of human breast cancer. Since the lCFs are associated with specific binding proteins (ICFBPs) which may modulate receptor/ligand interactions, production of IGFBPs by breast cancer cells could alter their IGF-dependent growth. This study examined the expression of ICFBPs 4, 5, and 6 in eight breast cancer cell lines (BCCLs) using ribonuclease (RNase) protection assays. IGFBP-4 mRNA was detected in all BCCLs studied. IGFBP-5 expression was higher in estrogen receptor (ER) positive cells, while IGFBP-6 mRNA was detected in only two ER negative BCCLs. We also found that E2 treatment enhanced the expression of IGFBPs 2, 4, and 5 in T47-D cells. We next studied IGFBP mRNA expression in 40 primary breast tumors. All tumors expressed mRNA for IGFBPs 2-6 but none expressed IGFBP-1 message. IGFBP-3 expression was higher in ER negative tumors, while that of IGFBP-4 and -5 was higher in ER positive specimens. These differences were statistically significant (P < .05). Ligand blot analysis of tumor extracts confirmed the presence of IGFBPs in breast cancer tissues. Thus, differential IGFBP expression in ER positive and negative tumors suggests an important role for this protein in breast cancer biology.

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