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Expression of human placental-type alkaline phosphatase in primary breast cancer

✍ Scribed by I. W. McDicken; G. H. Stamp; P. J. McLaughlin; P. M. Johnson

Publisher
John Wiley and Sons
Year
1983
Tongue
French
Weight
734 KB
Volume
32
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A monoclonal antibody (H317) with specificity for the heat‐stable placental‐type alkaline phosphatase (Pl‐ALP) isoenzyme has been used to investigate the occurrence of Pl‐ALP in patients with primary breast carcinoma. All pre‐operative plasma samples were negtive for Pl‐ALP in a sensitive solid‐phase enzyme immunoassay with a lower limit of detection of 0.1 U/I. In contrast, using a peroxidase‐anti‐peroxidase staining technique on fixed tissue sections, as well as enzyme immunoassay on fresh tissue extracts, Pl‐ALP could be demonstrated in the carcinomatous tissue of all seven patients investigated.

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