Expression of hepatocyte nuclear factor-3α in rat prostate, seminal vesicle, and bladder

✍ Scribed by Will Kopachik; Simon W. Hayward; Gerald R. Cunha

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 342 KB
• Volume: 211
• Category: Article
• ISSN: 1058-8388
• DOI: 10.1002/(sici)1097-0177(199802)211:2<131::aid-aja2>3.0.co;2-i

✦ Synopsis

Hepatocyte nuclear factor-3␣ (HNF-3␣), a member of the hepatocyte-forkheadhomolog family of transcription factors, regulates gene expression in the endoderm-derived liver and lung. To determine if HNF-3␣ might also play a role in endodermal derivatives of the urogenital sinus, the expression of HNF-3␣ in male accessory sex organs was assessed by Northern blotting, in situ hybridization, and electrophoretic mobility shift analysis. RNA from the dorsolateral prostate (DP), ventral prostate (VP), anterior prostate (AP), seminal vesicle (SV), and bladder was compared with RNA from the liver and spleen as positive and negative controls, respectively. HNF-3␣ mRNA levels in the DP, VP, AP, and bladder were 20, 14, 5, and 6 times higher than the SV equivalent in the liver. HNF-3␣ mRNA was detected in 8 of 10 prostate epithelial cell lines (rat NRP 152 and 154, mouse Pr14, and human DU-145, PC3, LNCaP, ND-1, and BPH-1) but not in rat Dunning epithelial or mouse Pr12 cells. Addition of testosterone to castrated rats was found to prevent a drastic loss of HNF-3␣ mRNA in the VP. This result suggests that HNF-3␣ mRNA levels are at least indirectly regulated by testosterone. The HNF-3␣ mRNA is expressed in epithelial cells of the urogenital sinus derivatives VP, AP, DP, and bladder and Wolffian duct derivative, the SV. To confirm that functional HNF-3␣ protein is produced in the VP, electrophoretic mobility shift assays were performed with wholecell extracts and high-affinity oligonucleotide (TTR-S) from the transthyretin promoter. Binding to TTR-S was disrupted when the extract was incubated with HNF-3␣, but not with HNF-3␤, antibody. Taken together, the results using VP, AP, DP, SV, and bladder suggest that HNF-3␣ may play an important role in development and maintenance of urogenital tract epithelial cells. Dev.