𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion

✍ Scribed by R. Daniel Bonfil; Paula A. Medina; Daniel E. Gómez; Eduardo Farias; Alberto Lazarowski; M. Fernanda Lucero Gritti; Roberto P. Meiss; Oscar D. Bustuoabad

Publisher
Springer
Year
1992
Tongue
English
Weight
820 KB
Volume
10
Category
Article
ISSN
0262-0898

No coin nor oath required. For personal study only.

✦ Synopsis

We have previously observed that acellular extracts from necrotic areas (NE) of the non-metastatic murine mammary adenocarcinoma M3, enhance in vitro cell detachment and spontaneous lung metastases. In the present study, using different proteinase inhibitors along with NE, only the calcium chelator EDTA could significantly abrogate the enhanced cell detachment from M3 produced by NE. The typical cleavage products of type IV collagenase were detected inside the tumor necrotic area, mainly in association with necrobiotic cells, as evaluated by Western blot analysis and immunohistochemical assays. Zymography revealed the presence of 72- and 92-kDa gelatinase/type IV collagenase in NE. Moreover, NE increased the in vitro invasive ability of cultured M3 cells. The use of specific antibodies against both 72- and 92-kDa type IV collagenases in the invasion assay showed that only the latter was able to revert the enhanced invasiveness to the baseline. It can be concluded that tumor necrosis is an important source of gelatinase/type IV collagenase, mainly in its 92 kDa form, and plays a major role in tumor invasion.

📜 SIMILAR VOLUMES

High expression of cathepsin B in transi
High expression of cathepsin B in transitional bladder carcinoma correlates with tumor invasion
✍ Ana M. Eiján; Eduardo O. Sandes; María D. Riveros; Sebastián Thompson; Leonardo 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 199 KB

## Abstract ## BACKGROUND Cathepsin B (CB) is a lysosomal cysteine proteinase synthesized as a zymogen of 39–47 kilodaltons (kD), which is subsequently converted into an active single‐ chain form of 33 kD (CB~33~) and, by additional processing, into the active 2‐chain form containing a heavy chain

Decreased expression of thrombomodulin i
Decreased expression of thrombomodulin is correlated with tumor cell invasiveness and poor prognosis in nonsmall cell lung cancer
✍ Po-Len Liu; Jong-Rung Tsai; Chien-Chih Chiu; Jhi-Jhu Hwang; Shah-Hwa Chou; Chih- 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB

## Abstract Thrombomodulin (TM) plays a role in coagulation, inflammation, and cell adhesion. Reduction of TM expression plays an important role in the tumor metastatic process; however, insufficient information is available regarding the expression of TM in nonsmall cell lung cancer (NSCLC). Sixty

Distinct progression-associated expressi
Distinct progression-associated expression of tumor and stromal MMPs in HaCaT skin SCCs correlates with onset of invasion
✍ Silvia Vosseler; Wiltrud Lederle; Kristiina Airola; Eva Obermueller; Norbert E. 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 888 KB

## Abstract Matrix metalloproteinases (MMPs) are critically involved in tumor invasion and metastasis. However, failure of broad spectrum MMP inhibitors in clinical trials emphasizes the need for detailed analyses of the specific role of different MMPs in tumor malignancy. Using HaCaT‐keratinocyte

Expression of matrix metalloproteinase 9
Expression of matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) induced by tumour necrosis factor α correlates with metastatic ability in a human osteosarcoma cell line
✍ A. Kawashima; I. Nakanishi; Y. Okada; H. Tsuchiya; A. Roessner; Ken'ichi Obata 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 1015 KB

We have examined the correlation between matrix metalloproteinase (MMP) expression and metastatic properties of a low metastatic osteosarcoma cell line, osteosarcoma takase (OST), under stimulation by tumour necrosis factor a (TNF~). In vivo, OST cells exhibited significantly increased colonization