Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry: a new tool in the analysis of the cell cycle
โ Scribed by J. Gong; X. Li; F. Traganos; Z. Darzynkiewicz
- Book ID
- 114716041
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 955 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0960-7722
No coin nor oath required. For personal study only.
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Background: Flow cytometry of immunofluorescence and DNA content provides measures of cell-cycle-related gene expression (protein and/or epitope levels) for asynchronously growing cells. From these data, time-related expression through S phase can be directly measured. However, for G1, G2, and M pha
## Phosphorylation of histone H3 (H3) on Ser-10 correlates with chromatin condensation at mitosis. A new monoclonal antibody (anti-H3-P) was developed that recognizes phosphorylated H3 (H3-P). This antibody was used in multiparameter flow cytometric analysis to relate H3 phosphorylation in individua