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Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors

✍ Scribed by Goshi Ishihara; Mie Goto; Mihoro Saeki; Kaori Ito; Tetsuya Hori; Takanori Kigawa; Mikako Shirouzu; Shigeyuki Yokoyama


Book ID
116828064
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
725 KB
Volume
41
Category
Article
ISSN
1046-5928

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## Abstract Herein we describe the methods for selective and reversible regulation of gene expression using antisense oligodeoxynucleotides (ODNs) in a cell‐free protein synthesis system programmed with multiple DNAs. Either a complete shut down or controlled level of gene expression was attained t