Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) inEscherichia coliand organ-related alteration of NDK activities during rice seed germination (Oryza sativaL.)
✍ Scribed by Akira Yano; Masaaki Umeda; Hirofumi Uchimiya
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 715 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
The GST (glutathione _S-transferase)-NDK (nucleoside diphosphate kinase) fusion protein was expressed in Escherichia coll. The GST-NDK protein was capable of transferring y-phosphate from ATP to nucleoside diphosphates such as GDP, CDP, TDP and UDP.
Western blot analysis using anti-NDK antibody indicated that NDK in endosperm gradually decreased during 36 h of imbibition. On the contrary, NDK in embryo increased during the same period. NDK activities in both tissues were in accord with these observations. Whereas the NDK protein in roots of rice seedlings during 7 days of imbibition remained constant, in shoots it declined after 5 days of imbibition. Thus, NDK may play a significant role in the cellular event modulated by adenylate energy charge level.