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Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme

✍ Scribed by Ahmed E. Fazary; Hamed A. Hamad; John C. Lee; Takuya Koskei; Cheng-Kang Lee; Yi-Hsu Ju


Book ID
116542257
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
471 KB
Volume
46
Category
Article
ISSN
0141-8130

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We engineered an acetyl xylan esterase (AwaxeA) gene from Aspergillus awamori into a heterologous expression system in Pichia pastoris. Purified recombinant AwAXEA (rAwAXEA) displayed the greatest hydrolytic activity toward alpha-naphthylacetate (C2), lower activity toward alpha-naphthylpropionate (