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Expression of Escherichia coli promoters in Brevibacterium lactofermentum using the shuttle vector pEB003

✍ Scribed by Morinaga, Yasushi; Tsuchiya, Makoto; Miwa, Kiyoshi; Sano, Konosuke


Book ID
123254751
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
497 KB
Volume
5
Category
Article
ISSN
0168-1656

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To determine the utility of coupling runaway replication to the expression of cloned genes under the control of strong promoters, lacZ transcriptional fusions to the trp or tac promoter (Ptrp or Ptac) were constructed using plasmids in which the copy number is thermally regulated. Cells containing t