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Expression of epstein-barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

✍ Scribed by Su Ling Li; H. Feichtinger; E. Kaaya; P. Biberfeld; P. Migliorini; P. Putkonen; G. Biberfeld; J. M. Middeldorp; I. Ernberg

Publisher
John Wiley and Sons
Year
1993
Tongue
French
Volume
55
Category
Article
ISSN
0020-7136

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✦ Synopsis

Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one

third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in I3 cyno-EBVcarrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these turriors. lmmunoblots of cell tysates were probed with polyrpecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMP I. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/ 13) and in the 3 cell linas derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAl . Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPl could not be detected. Immunophenotypicai characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

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