Expression of embryonic and adult erythrocyte antigens in hematopoietic repopulated x-irradiated chickens
✍ Scribed by Sanders, Bob G. ;Wiley, K. L.
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 420 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
A n antigen is present on the red blood cells of embryonic and young chickens which is lost during development and is undetectable on the red blood cells of adult chickens. In this study, two experiments were performed to determine the presence or absence of the embryonic antigen. First, spleen, liver and bone marrow cells obtained from three day old chicks were transplanted into x-irradiated adult chickens i n order to determine if the embryonic antigen would be expressed on red blood cells in a n adult environment but arising from transplanted "young" cells. Red blood cells from the adult x-irradiated chickens were assayed at various intervals after transplantation for the presence of the embryonic antigen. Additional red blood cell antigenic markers other than the embryonic antigen demonstrated repopulation i n the x-irradiated chickens, but the embryonic antigen was not detectable. Second, three day old, non-irradiated chickens were transplanted with adult chicken bone marrow cells and their red blood cells assayed at varying intervals up to 40 days in order to determine the effect of a "young" environment upon adult cells. These chickens continued to express the embryonic antigen on all their red blood cells; i.e., no adult-like cells were found up to 40 days. The x-irradiation-transplantation studies indicate that the erythropoietic stem cells containing gene(s) coding for the embryonic antigen are repressed i n the adult chicken and that i n the young chicken repopulated with adult hemopoietic cells the gene(s) are derepressed.