✦ LIBER ✦

Expression of desiccation-related proteins from the resurrection plantCraterostigma plantagineumin transgenic tobacco

✍ Scribed by Gabriel Iturriaga; Katharina Schneider; Francesco Salamini; Dorothea Bartels

Publisher: Springer
Year: 1992
Tongue: English
Weight: 381 KB
Volume: 20
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00040614

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✦ Synopsis

Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3'-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.

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