Expression of cytochrome P-450lpr Is developmentally regulated and limited to house fly

✍ Scribed by Wheelock, Geoffrey D. ;Konno, Yasuhiko ;Scott, Jeffrey G.

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 687 KB
• Volume: 6
• Category: Article
• ISSN: 0887-2082
• DOI: 10.1002/jbt.2570060402

✦ Synopsis

Expression of house fly cytochrome P-45OIpr was examined using immunoblotting in male and female adult LPR house flies, mixed sex adult house flies at 12 different ages, larvae, and pupae. P-45OlPr was expressed in both male and female adult house flies. P-45OIpr was clearly present in all adult stages examined, was barely detectable in pupae, and could not be detected in larvae. Thus, cytochrome P-45OlPr is developmentally regulated and present in both sexes of house fly.

Expression of cytochrome P-450, immunologically homologous to house fly cytochrome P-45OIp,, was examined in other species using immunoblot analysis. Eleven animal species were tested in the orders Diptera, Hymenoptera, Lepidoptera, Orthoptera, Acari, and Rodentia, using microsomes in some species from both induced and noninduced animals or insecticideresistant and susceptible strains. P-45OIp, appears to be restricted to house flies, as none of these species contained cytochrome P-450 that reacted with antiserum to cytochrome P-45OIp,.