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Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems

✍ Scribed by Marianne Wittrup Larsen; Uwe T. Bornscheuer; Karl Hult

Book ID: 116828868
Publisher: Elsevier Science
Year: 2008
Tongue: English
Weight: 300 KB
Volume: 62
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2008.07.012

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led