✦ LIBER ✦

Expression of brush border enzymes in response to lead exposure in rat intestine

✍ Scribed by Priya Kapur; Kamaljit Kaur; Akhtar Mahmood

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 117 KB
Volume: 25
Category: Article
ISSN: 0260-437X
DOI: 10.1002/jat.1079

No coin nor oath required. For personal study only.

✦ Synopsis

The effect of feeding lead (50 mg kg(-1) body weight) daily for 7 days on the development of various brush border enzymes in the intestine has been studied. The activities of brush border sucrase (P < 0.001), lactase (P < 0.001), gamma-glutamyl transpeptidase (P < 0.05) and leucine aminopeptidase were reduced (P < 0.05), whereas the alkaline phosphatase level was augmented (P < 0.05) in lead fed rats compared with controls. Kinetic studies with sucrase revealed a low Vmax (0.224 in control and 0.160 units mg(-1) protein in lead exposed) with no change in Km (12.6-13.5 mM). Western blot analysis for alkaline phosphatase yielded intense staining of enzyme protein in lead fed rats compared with controls, however, the intensity of the antigen signal was reversed for sucrase under these conditions. These findings suggest that ingestion of lead may interfere with the crypt cell differentiation process thus affecting enzyme functions in the rat intestine.

📜 SIMILAR VOLUMES

Alterations in the expression of intesti

Alterations in the expression of intestinal enzymes in rats exposed to nickel

✍ Amika Singla; Jaspreet Kaur; Akhtar Mahmood 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 142 KB 👁 1 views

## Abstract The effect of feeding nickel (50 mg kg^−1^ body weight) daily for 7 days was studied on the development of various brush border enzymes across the crypt–villus axis. The activities of brush border maltase (__P__ < 0.05), lactase (__P__ < 0.05), alkaline phosphatase (__P__ < 0.05) and le

Lack of renal secretion of carbenicillin

Lack of renal secretion of carbenicillin in rats: poor affinity to the organic anion transporter at renal brush border membrane

✍ Tomoo Itoh; Shin-ichi Koyano; Hiromi Nakaura; Yasuyuki Tsuda; Hideo Yamada 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 206 KB 👁 2 views

The renal secretion of carbenicillin (CBPC) was studied in rats. The results obtained in the in vivo study indicated very poor renal secretion of CBPC in rats, which was entirely different from those observed in humans and rabbits. In humans and rabbits, significant and stereoselective renal secreti

Transcriptomic analysis of intestinal fi

Transcriptomic analysis of intestinal fibrosis-associated gene expression in response to medical therapy in Crohn's disease

✍ John P. Burke; Marc Ferrante; Karen Dejaegher; R. William G. Watson; Neil G. Doc 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 184 KB

Background: Glucocorticoids and monoclonal antibodies to tumor necrosis factor reduce inflammation in Crohn's disease (CD). Rapid luminal healing, however, may promote intestinal stricture formation. The aim of this study was to examine fibrosis-associated gene expression in the intestine of patient

Effect of prenatal and postnatal exposur

Effect of prenatal and postnatal exposure to lead on kidney function in male and female rats

✍ A. Vyskocil; M. Cizkova; I. Tejnorova 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 186 KB 👁 2 views

The effect of 5 months' exposure to 0.5% lead acetate in drinking water on the kidney function of developing rats was studied. In both sexes, lead exposure produced a significant elevation of the kidney weight and after 3 months' treatment both male and female rats showed signs of tubular impairment

Expression of rat renal cortical OAT1 an

Expression of rat renal cortical OAT1 and OAT3 in response to acute biliary obstruction

✍ Anabel Brandoni; Silvina R. Villar; Juan C. Picena; Naohiko Anzai; Hitoshi Endou 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 408 KB 👁 1 views

Renal function in the course of obstructive jaundice has been the subject of great interest; however, little is known about the expression of renal organic anion transporters. The objective of this work was to study, in rats with acute extrahepatic cholestasis, the cortical renal expression of the o

Regional distribution of neuropeptide-de

Regional distribution of neuropeptide-degrading enzyme activity in the rat brain: Effects of subacute exposure to carbon disulfide

✍ De Gandarias, J. M. ;Echevarría, E. ;Irazusta, J. ;Casis, O. ;Casis, L. 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 489 KB

Carbon disulfide, a volatile solvent, is widely used in industry. It has been demonstrated that it causes several neuropsychological symptoms. However, the neurochemical basis of its neurotoxic effect is relatively unknown. In this paper we have measured the effect of subacute i.p. administration on