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Expression of bovine pancreatic ribonuclease A coded by a synthetic gene in Bacillus subtilis

✍ Scribed by Vasantha, N.; Filpula, David


Book ID
121778481
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
794 KB
Volume
76
Category
Article
ISSN
0378-1119

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Expression of the Bacillus subtilis polC
✍ Ott, Russell W. ;Goodman, Laurie E. ;Ganesan, A. T. πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 798 KB

We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell