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Expression of an Escherichia coli flagellin gene, hag48, in the presence of a Salmonella H1-repressor

โœ Scribed by Enomoto, Masatoshi ;Sakai, Akiko ;Tominaga, Akira

Publisher
Springer
Year
1985
Tongue
English
Weight
345 KB
Volume
201
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

An Escherichia coli K12 flagellin gene, hag48, was found to be expressed in the presence of the Salmonella rhl gene product. The strains which had hag48 on a chromosome or on an F' factor were constructed from strains H2-e,n,x .... ff rhl + and fixed H2-e,n,x ยฐ" rhl + in which rhl + is cotranscribed with H2 in its "on" state. Motility of these strains in semisolid medium was inhibited by anti-H48 serum and motile clones (swarms) that escaped from it were hag mutants in case of the hag48 e,n,x ยฐn-ยฐff strain tested. H48 flagellin was detected by electrophoresis, though its amount was less than e,n,x flagellin, from all the strains that were nonmotile in the presence of anti-H48 serum.

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