Expression of adenosine A1 receptors in cultured neurons from fetal rat brain

✍ Scribed by Frédéric Nicolas; Jean-Luc Daval

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 478 KB
• Volume: 14
• Category: Article
• ISSN: 0887-4476
• DOI: 10.1002/syn.890140113

✦ Synopsis

Abstract

The expression of adenosine A~1~ receptors was investigated using [^3^H]2‐chloro‐N^6^ ‐cyclopentyladenosine (CCPA) in 8‐day‐old cultured neurons from fetal rat forebrain grown in serum‐free medium. [^3^H]CCPA bound specifically and with high affinity (Kd = 2.9 nM) to a homogeneous population of sites. Displacement of CCPA binding by various adenosine derivatives indicated that A~1~ receptors were selectively labeled. The presence of Gpp(NH)p, a GTP analogue, reduced significantly the binding affinity (Kd = 12.2 nM), suggesting that A~1~ receptors detected in intact cultured cells are linked to associated G proteins. © 1993 Wiley‐Liss, Inc.