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Expression of a novel tropomyosin isoform in axolotl heart and skeletal muscle

✍ Scribed by Anish Thomas; Sudarsan Rajan; Harold L. Thurston; Sreeharsha N. Masineni; Preeti Dube; Abhishek Bose; Vasundhara Muthu; Syamalima Dube; David F. Wieczorek; Bernard J. Poiesz; Dipak K. Dube

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
405 KB
Volume
110
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

TPM1ΞΊ is an alternatively spliced isoform of the TPM1 gene whose specific role in cardiac development and disease is yet to be elucidated. Although mRNA studies have shown TPM1ΞΊ expression in axolotl heart and skeletal muscle, it has not been quantified. Also the presence of TPM1ΞΊ protein in axolotl heart and skeletal muscle has not been demonstrated. In this study, we quantified TPM1ΞΊ mRNA expression in axolotl heart and skeletal muscle. Using a newly developed TPM1ΞΊ specific antibody, we demonstrated the expression and incorporation of TPM1ΞΊ protein in myofibrils of axolotl heart and skeletal muscle. The results support the potential role of TPM1ΞΊ in myofibrillogenesis and sarcomeric function. J. Cell. Biochem. 110: 875–881, 2010. Β© 2010 Wiley‐Liss, Inc.

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