Expression of a dominant negative IκB-α modulates hypersensitivity of ataxia telangiectasia fibroblasts to streptonigrin-induced apoptosis
✍ Scribed by Mira Jung; Yin Zhang; Anatoly Dritschilo
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 124 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1065-7541
No coin nor oath required. For personal study only.
✦ Synopsis
Ataxia telangiectasia (AT) cells exhibit greater levels of apoptosis than normal fibroblasts following exposure to X-rays or radiomimetic drugs. In this study, we investigated apoptosis in AT cells whose radiation sensitivity has been altered by transfection with a cDNA expressing truncated IB-␣ (⌬IB-␣). ⌬IB-␣ functions as a dominant negative regulatory protein of NF-B. The transfected cells (ATCL11) were compared to parental cells after treatment with the radiomimetic drug streptonigrin. ATCL11 cells exposed to streptonigrin demonstrated less apoptosis (∼2%) at 24 hr than did parental AT cells (∼24%). These data indicate that the mechanisms underlying apoptosis induction by streptonigrin are modulated by regulation of NF-B.