Expression in bladder transitional cell carcinoma by real-time quantitative reverse transcription polymerase chain reaction array of 65 genes at the tumor suppressor locus 9q34.1-2: Identification of 5 candidates tumor suppressor genes
✍ Scribed by Najla Amira; Géraldine Cancel-Tassin; Stéphane Bernardini; Béatrix Cochand-Priollet; Hugues Bittard; Philippe Mangin; Georges Fournier; Alain Latil; Olivier Cussenot
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- French
- Weight
- 122 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Frequent deletions on 9q34.1‐2 were reported in bladder transitional cell carcinoma. High deletion mapping studies delimited a critical interval between markers D9S61 and D9S66, which is highly susceptible to contain a tumor suppressor gene. Expression level of the 65 genes localized in this region was analyzed by real‐time quantitative RT‐PCR, comparing tumor to normal urothelium. Five genes exhibited a significantly reduced expression level: C9orf9, KIAA0625, ABL1, LAMC3 and KIAA1857‐netrin‐G2, which exhibited the most significant downregulation (p=0.0007). KIAA1857‐netrin‐G2 belongs to the netrins and might then be a tumor suppressor gene in bladder cancer, as netrin1 receptor DCC has been implicated in tumorigenesis. © 2004 Wiley‐Liss, Inc.