Expression, Assembly, and Proteolytic Processing of Helminthosporium victoriae 190S Totivirus Capsid Protein in Insect Cells

Self-assembly of the infectious bursal d

Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography

✍ Min-Ying Wang; Yung-Yan Kuo; Meng-Shiou Lee; Shyue-Ru Doong; Ji-Yi Ho; Long-Huw 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

A gene encoding a structural protein (VP2) of a local strain (P3009) of infectious bursal disease virus (IBDV) was cloned and expressed using the baculovirus expression system to develop a subunit vaccine against IBDV infection in Taiwan. The expressed rVP2 proteins formed particles of approximately