The t'mk of this work was the est'ablishment of an effect,ive t'ransfer system for F'-plasniids from Esc1,eticlkin coli to Pr0teu.s mircrbilis. It. is shown t,liat cellfi of PG VI act as recipients in crosses wit,li E. coli F' st'rains but wit'h a low transfer rate of the plasmid. The presumption th
Expression and stability of Escherichia coli F-prime factors in Proteus mirabilis
✍ Scribed by Morgan, Edward A. ;Kaplan, Samuel
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 621 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
The expression and stability of Escherichia coli F-primes in Proteus mirabilis is examined. It is possible to consecutively introduce, and stably maintain, the DNA of several E. coli F-primes in P. mirabilis in the absence of selective pressure for all or some of the plasmids. Additionally, we can recover more than one F-prime from certain P. mirabilis recipient strains which carry DNA derived from several independent matings with E. coli F-prime donors.
📜 SIMILAR VOLUMES
## Abstract L‐amino acid deaminases catalyze the deamination of natural L‐amino acids. Two types of L‐amino acid deaminase have been identified in __Proteus__ species. One exhibits high levels of activity toward a wide range of aliphatic and aromatic L‐amino acids, typically L‐phenylalanine, wherea
The hemolytic activity of Escherichia coli and Proteus vulgaris is determined by common contiguous genes encoding synthesis (hly C, hly A) and specific secretion (hly B, hly D) of active hemolysin. Nevertheless, the hly C-proximal DNA sequences directing production of the homologous hemolysins by th
A number of spontaneous rifampicin-resistant (RIP) mutants were isolated from a strain of E. coli having a deletion in the lac proA proB region of the chromosome. The stability of a F'lac proA proB episome in these mutants was determined by their sensitivity to acridine orange curing and the frequen