𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression and regulation of steroid 5α-reductase in the genital tubercle of the fetal rat

✍ Scribed by Hui Tian; David W. Russell

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
594 KB
Volume
209
Category
Article
ISSN
1058-8388

No coin nor oath required. For personal study only.

✦ Synopsis

The genital tubercle is a bipotential anlage that can develop into a penis or a clitoris. Formation of the penis requires synthesis within the tubercle of the androgen dihydrotestosterone by steroid 5 alpha-reductase isozymes. Isozyme 1 mRNA and protein were present in epithelial cells, whereas isozyme 2 transcripts were present in mesenchymal cells of developing genital tubercles from day 17, 19, and 21 rat embryos. These expression patterns were observed in both uncommitted anlagen and in committed male or female structures. Androgens did not significantly influence levels of 5 alpha-reductase mRNAs or affect their cellular distributions. Together with genetic data, the results implicate the mesenchyme as the crucial cell type in formation of the male external genitalia.

📜 SIMILAR VOLUMES

Identification and partial characterizat
Identification and partial characterization of two steroid 5α-reductase isozymes in the canine prostate
✍ Span, Paul N.; Schalken, Jack A.; Sweep, Fred G.J.; Smals, Anthony G.H. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 1 views

## BACKGROUND. The dog has been extensively used as an in vivo model to test the pharmacokinetics and effects on pathological prostatic growth of 5␣-reductase inhibitors. However, no information is available on the existence or characteristics of canine 5␣-reductase isozymes. METHODS. The 5␣-reduc

Pharmacological and molecular evidence f
Pharmacological and molecular evidence for the expression of the two steroid 5α-reductase isozymes in normal and hyperplastic human prostatic cells in culture
✍ Berthaut, Isabelle; Mestayer, Chidi; Portois, Marie-Claire; Cussenot, Olivier; M 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 1 views

## BACKGROUND. Whereas the embryological development of the human prostate is clearly dependent on steroid 5␣-reductase (5␣-R) type 2 expression, the respective expression of the two known isoforms (types 1 and 2) of 5␣-R in the adult human prostate remains unclear. METHODS. 5␣-R isoform mRNA expr

Differential expression of 5α-reductase
Differential expression of 5α-reductase isoenzymes in the human prostate and prostatic carcinomas
✍ Bonkhoff, Helmut; Stein, Ute; Aumüller, Gerhard; Remberger, Klaus 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 658 KB

BACKGROUND. Steroid 5a-reductase is essential for the intracellular accumulation of dihydrotestosterone (DHT), which mediates androgen effects on target tissue. METHODS. In the present study, we describe the differential expression and cellular localization of 5a-reductase 1 and 2 isoenzymes in the

Molecular analysis of the 5α-steroid red
Molecular analysis of the 5α-steroid reductase type 2 gene in a family with deficiency of the enzyme
✍ Vilchis, F.; Canto, P.; Chávez, B.; Ulloa-Aguirre, A.; Méndez, J. P. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB 👁 1 views

This report describes the identification of a point mutation in the 5␣-reductase type 2 (5␣-SR2) gene from a family in which both sibs (6 and 3 years old) have steroid 5␣-reductase 2 deficiency. The five exons of the gene were individually amplified by the polymerase chain reaction (PCR) and analyse