Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)—characterization of its phosphatase activity and interaction with Golli-BG21

Purification and spectroscopic character

Purification and spectroscopic characterization of the recombinant BG21 isoform of murine golli myelin basic protein

✍ Vladimir V. Bamm; Mumdooh A.M. Ahmed; Vladimir Ladizhansky; George Harauz 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 691 KB

## Abstract A recombinant form of the murine Golli‐myelin basic protein (MBP) isoform BG21 (rmBG21) has been expressed in __E. coli__, and isolated to 96% purity via metal chelation chromatography. Characteristic yields were 6–8 mg protein per liter of culture in either minimal M9 or standard Luria