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Expression and purification of hepatitis B surface antigen S fromEscherichia coli; a new simple method

✍ Scribed by Mohamed S Elghanam, Ahmed S Attia, Hussein A Shoeb, Abd Elgawad M Hashem

Book ID
119912557
Publisher
BioMed Central
Year
2012
Tongue
English
Weight
677 KB
Volume
5
Category
Article
ISSN
1756-0500

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## Abstract We constructed a recombinant plasmid that can express the entire pre‐S2 sequence of hepatitis B surface antigen (HBsAg) as a fusion protein in __E. coli__. The hybrid protein, which comprises the bacterial TrpLE protein and the pre‐S2 sequence, was the prominent protein that was found i