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Expression and Purification of ATP Sulfurylase from Saccharomyces cerevisias in Escherichia coli and Its Application in Pyrosequencing

✍ Scribed by LUO, J; WU, W; ZOU, B; ZHOU, G


Book ID
122099568
Publisher
Elsevier Science
Year
2007
Weight
368 KB
Volume
23
Category
Article
ISSN
1872-2075

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The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a