Expression and purification of antimicrobial peptide CM4 by Nprofusion technology inE. coli
β Scribed by Xiawei Cheng; Wuguang Lu; Shuangquan Zhang; Peng Cao
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 432 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0939-4451
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A fusion protein was genetically engineered that contains an antimicrobial peptide, designated P2, at its carboxy terminus and bovine prochymosin at its amino terminus. Bovine prochymosin was chosen as the fusion partner because of its complete insolubility in Escherichia coli, a property utilized t
## Abstract A method is presented that allows efficient production of antimicrobial peptides in bacteria by means of fusion to the histone fold domain of the human transcription factor TAF12. This small fusion partner drives highβlevel expression of peptides and leads to their accumulation in an en