Expression and purification of a biologically active basic fibroblast growth factor fusion protein
โ Scribed by Zhi Sheng; Shin-Bey Chang; William J Chirico
- Book ID
- 117702608
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 183 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1046-5928
No coin nor oath required. For personal study only.
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A biologically active fusion protein comprising a short hydrophilic leader peptide fused to the N-terminus of rat CNTF was generated using commercially available materials. The coding region for rat CNTF was sub-cloned into the pFLAG-1 vector and transfected into the JM 109 strain of E. coli. The tr